GHRP-6 Acetate (5mg)

Description

GHRP-6 Acetate – Advanced Biochemical Mechanism & Pathway Profile

(Growth Hormone Releasing Peptide-6 – Hexapeptide Ghrelin-Mimetic)

GHRP-6 is a synthetic hexapeptide classified as a growth hormone secretagogue. It acts as an agonist at the Growth Hormone Secretagogue Receptor type 1a (GHS-R1a), a G-protein–coupled receptor that also mediates effects of endogenous ghrelin. The acetate salt is used for formulation stability in research environments.

✅ Primary Receptor Target

• GHS-R1a (GPCR, predominantly Gq and Ghs coupled)

• Expressed in pituitary somatotrophs and hypothalamic neurons

✅ Core Intracellular Signaling Cascades

1. Gq → PLCβ Pathway

GHRP-6 binding to GHS-R1a activates Gq, initiating:

1. Phospholipase C-β (PLCβ) activation

2. PIP2 → IP3 + DAG hydrolysis

3. IP3-mediated Ca²⁺ release from the endoplasmic reticulum

4. DAG → PKC activation

This calcium-dependent mechanism stimulates vesicular GH release.

Key molecular outputs studied:

• ↑ intracellular Ca²⁺

• PKC phosphorylation events

• Activation of calcium-dependent transcription factors

2. Ghs/Gi → cAMP Modulation

GHS-R1a can couple to Ghs (ghrelin-specific G-proteins) or Gi:

• Altered adenylate cyclase signaling

• Changes in cAMP → PKA activity

• cAMP-dependent phosphorylation of transcription factors such as CREB

3. Ghrelin-Like Neuropeptide Signaling

GHRP-6 stimulates hypothalamic neurons that control pituitary output.

Associated pathways of interest:

• NPY/AgRP neuron activation

• AMP-activated protein kinase (AMPK) signaling in metabolic nuclei

• Calcium-dependent neurotransmitter release

✅ Gene-Level Targets Studied

Although GHRP-6 itself does not bind DNA or nuclear receptors, downstream endocrine signaling alters transcription of:

✅ Additional Pathways Observed In-Vitro

MAPK/ERK Cascade

Activation of GHS-R1a can trigger:

• Ras → Raf → MEK1/2 → ERK1/2

• Gene transcription linked to somatotroph responsiveness and vesicle biogenesis

Calcium-Dependent Exocytosis

GH secretory granule release is driven by:

• Ca²⁺ influx via voltage-gated channels

• SNARE-complex membrane fusion

• PKC-regulated synaptotagmin proteins

✅ Summary of Key Molecular Targets

Research-Only Classification

GHRP-6 Acetate is provided exclusively for in-vitro laboratory research.
It is not approved for human or animal use, ingestion, injection, or diagnostic or therapeutic application.