ARA-290 (10mg)

Description

ARA-290 (Cibinetide) – Advanced Biochemical Mechanism & Signaling Profile

Erythropoietin-Derived Helix-B Peptide

ARA-290 is a synthetic 11–amino-acid peptide derived from the B-helix region of erythropoietin (EPO). Unlike full-length EPO, ARA-290 does not activate the classical EPO receptor homodimer involved in erythropoiesis.
Instead, research demonstrates selective binding to the Innate Repair Receptor (IRR), a heterodimer composed of:

• EPO Receptor (EPOR) + CD131 (β-common receptor)

This selective activity allows study of cytoprotective and anti-inflammatory signaling independent of erythropoietic pathways.

✅ Primary Signaling Pathways

Binding of ARA-290 to the EPOR–CD131 IRR complex activates intracellular kinase signaling cascades:

1. JAK2/STAT3 Pathway

• IRR engagement → JAK2 phosphorylation

• JAK2 phosphorylates STAT3 and STAT5

• STATs translocate to the nucleus to regulate target genes

Gene targets studied:

• SOCS3 (feedback inhibitor of cytokine signaling)

• Bcl-xL (cell-survival protein)

• Heme oxygenase-1 (HO-1)

• Antioxidant response genes

2. PI3K/Akt Pathway

• PI3K activation → PIP3 generation

• Recruitment and phosphorylation of Akt (Ser473, Thr308)

• Akt influences mitochondrial survival signaling, apoptosis suppression, and metabolic regulation

Downstream influences:

• GSK-3β phosphorylation (inactivation)

• mTOR modulation (cellular stress & repair models)

• FOXO transcription factors

3. NF-κB Modulation

ARA-290 has been studied for suppression of:

• IKK activity

• NF-κB nuclear translocation

• Pro-inflammatory gene transcription

Genes negatively regulated in models:

• TNF-α

• IL-1β

• IL-6

• MCP-1 (CCL2)

✅ Enzymes & Molecular Targets

Research has examined effects on:

✅ Ion Channels & Neuroimmune Targets

Some research models show ARA-290 interacting with:

• TRPV1 & TRPA1 modulation (nociception studies)

• Macrophage phenotype switching (M1 → M2)

• Microglial signaling alterations

These findings position ARA-290 in models of inflammatory and neuropathic signaling biology.

✅ Why ARA-290 Is Distinct From Erythropoietin

This allows researchers to isolate non-hematopoietic EPO-derived signaling.

Research-Only Classification

ARA-290 is supplied solely for laboratory and in-vitro scientific research.
It is not a drug, supplement, therapeutic agent, or diagnostic material, and is not approved for human or animal use.