8X Blend L-Carnitine (20mg) / L-Arginine (20mg) / Methonine (25mg) / Inositol (50mg) / Choline (50mg) / B6 (25mg) / B5 (5mg) / B12 (1mg)

Description

8X Nutrient Research Blend – Advanced Biochemical Profile

L-Carnitine (20mg) / L-Arginine (20mg) / Methionine (25mg) / Inositol (50mg) / Choline (50mg) / B6 (25mg) / B5 (5mg) / B12 (1mg)

This blend features eight biochemically active compounds frequently examined in models of mitochondrial metabolism, methylation cycles, lipid processing, and coenzyme-dependent enzymatic reactions. Each component interacts with distinct metabolic pathways and downstream gene targets.

L-Carnitine

Primary Pathway: Fatty-Acid β-Oxidation Shuttle
Key Enzymes:

• CPT-I (Carnitine Palmitoyltransferase I) – catalyzes acyl-carnitine formation

• CACT (Carnitine-Acylcarnitine Translocase) – mitochondrial transport

• CPT-II – conversion back to acyl-CoA inside mitochondria

Research Focus:

• Mitochondrial respiration

• ATP production during lipid oxidation

• PPAR-α regulated genes (CPT1A, ACADs, ACOX1)

L-Arginine

Primary Pathways: Nitric-Oxide Cycle & Urea Cycle
Key Enzymes:

• NOS (Nitric Oxide Synthase) → conversion to NO and citrulline

• ASS/ASL (Argininosuccinate Synthetase/Lyase) – urea-cycle intermediates

Downstream Signaling:

• cGMP signaling via guanylate cyclase activation

• Studies of vascular and metabolic gene regulation associated with NO pathways

Methionine

Primary Pathways: Methionine Cycle → Trans-Sulfur Pathway
Key Enzymes:

• MAT – conversion to SAMe

• Met Synthase / BHMT – homocysteine remethylation

• CBS/CSE – cysteine & glutathione synthesis

Gene Targets Studied:

• DNA methylation status of CpG islands

• Expression of methylation-sensitive genes

• Glutathione-related oxidative-stress markers

Inositol

Primary Pathway: Phosphatidylinositol (PI) Cycle
Key Enzymes:

• PI-Kinases → PIP2/PIP3 formation

• PLC → IP3 and DAG second messengers

• PKC & Ca²⁺ signaling downstream

Cellular Research Uses:

• Membrane signaling

• Insulin pathway modeling

• IP3-mediated calcium release and gene expression

Choline

Primary Pathways:

1. Phosphatidylcholine Synthesis (Kennedy Pathway)

   • Enzymes: Choline Kinase, CCT, CPT

2. Betaine-Homocysteine Methylation

   • Enzyme: BHMT

Gene Targets Studied:

• Lipid export (ApoB100, VLDL secretion)

• Membrane phospholipid turnover

• Methyl-donor gene interactions

Vitamin B6 (Pyridoxine)

Primary Pathway: Amino-Acid Metabolism
Coenzyme Form: PLP (Pyridoxal-5-Phosphate)
Enzymes:

• Transaminases (AST/ALT)

• Cystathionine β-synthase in methionine cycle

• Decarboxylases for neurotransmitter synthesis

Research Relevance:

• Neurochemical synthesis genes

• Homocysteine metabolism

• Amino-acid turnover studies

Vitamin B5 (Pantothenic Acid)

Primary Pathway: Coenzyme A Biosynthesis
Key Enzymes: PANK, PPCS, PPCDC, PPAT, DPCK

Downstream:

• Acetyl-CoA production

• TCA cycle input via PDH complex

• Fatty-acid synthesis and β-oxidation

Vitamin B12 (Cobalamin)

Key Enzymes:

• Methionine Synthase (MS) – converts homocysteine → methionine

• Methylmalonyl-CoA Mutase

Research Focus:

• DNA synthesis and cell division

• Myelin and neurological biochemical models

• Methylation and carbon-flow metabolism

Integrated Biochemical Interest

Researchers use this blend to examine:

• Methyl-donor flux and homocysteine regulation

• β-oxidation and mitochondrial ATP generation

• CoA-dependent enzymatic function

• Phosphatidylinositol second-messenger signaling

• Nitric-oxide–mediated cGMP pathways

• Epigenetic and transcriptional responses to methyl availability

Research-Only Classification

This product is supplied solely for laboratory and in-vitro scientific research.
It is not approved for human or animal use, clinical application, or diagnostic purposes.