4X Blend GHRP-2 (5mg) / Tesamorelin (5mg) / MGF (500mcg) / Ipamorelin (2.5mg)

Description

4X Peptide Blend – Advanced Biochemical Mechanism Overview

GHRP-2 (5mg) / Tesamorelin (5mg) / MGF (500mcg) / Ipamorelin (2.5mg)

This blend comprises four peptides frequently utilized in advanced endocrine and cellular-signaling models. Together, these agents engage both GHS-R and GHRH-R pathways, as well as IGF-1R-mediated gene expression, enabling multi-axis analysis of growth-hormone and regenerative signaling.

GHRP-2

Primary receptor: GHS-R1a (growth hormone secretagogue receptor)
Signaling cascade:

• GHS-R1a → Gq/11 protein coupling

• Activation of phospholipase-C (PLC)

• Generation of IP3 and DAG second messengers

• Increased intracellular calcium release

• PKC activation

Downstream gene targets studied in vitro:

• GH1 (growth hormone gene transcription)

• c-Fos, c-Jun (immediate early genes)

• SOCS family for negative-feedback studies

• Calcium-dependent CREB phosphorylation

Research models frequently investigate GHRP-2 for its ability to augment pulsatile GH release while bypassing somatostatin inhibition in cultured pituitary cells.

Tesamorelin

Primary receptor: GHRH-R (Class B GPCR on pituitary somatotrophs)
Signaling cascade:

• GHRH-R → Gs coupling

• Adenylyl cyclase activation

• cAMP accumulation

• PKA activation

• Phosphorylation of CREB

Downstream transcriptional targets:

• GH1 and POU1F1 (Pit-1) for pituitary GH synthesis

• IGF-1 gene expression in hepatocyte models

• Feedback on hypothalamic somatostatin neurons

Tesamorelin’s stabilized structure provides extended receptor binding time, making it useful for controlled experiments requiring sustained cAMP-PKA activation.

MGF (Mechano Growth Factor – IGF-1 Ec isoform)

Primary receptor: IGF-1R (tyrosine kinase receptor)
Signaling cascade:

• IGF-1R autophosphorylation

• Recruitment of IRS-1/2

• Activation of PI3K→Akt→mTOR pathway

• MAPK/ERK cascade initiation

Downstream biological targets in research settings:

• Satellite cell activation markers (Pax7, MyoD)

• mTOR-regulated protein synthesis genes

• Cell-cycle progression markers in repair studies

MGF is often used in localized cell-culture models to compare autocrine/paracrine growth-factor signaling vs. systemic IGF-1 activity.

Ipamorelin

Primary receptor: GHS-R1a (selective ghrelin mimetic)
Signaling specificity:

• Highly selective for GH secretion with limited cross-activation of ACTH, prolactin, or cortisol-related pathways in lab studies

• Engages cAMP and PLC signaling without strong off-target endocrine effects

Second messengers & downstream targets:

• IP3-mediated Ca²⁺ mobilization

• PKC and CREB activation

• Studies on GH gene transcription modulation and receptor binding kinetics

Combined Signaling Interest

With both GHS-R1a and GHRH-R pathways stimulated simultaneously, researchers often use this blend to examine:

• Convergent signaling on GH1 transcription

• Cooperative effects on CREB-regulated gene expression

• IGF-1R-mediated mTOR activation following GH-axis stimulation

• Comparative receptor kinetics between ghrelin mimetics and GHRH analogs

• Cross-talk between MAPK/ERK and PI3K/Akt pathways in regenerative models

Research-Only Classification

This material is restricted to in-vitro and laboratory research.
It is not approved for human or animal use, clinical testing, or diagnostic procedures.